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 Cloning Kit new

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    Rabbit IgG SmartBlot

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  siRNA Products

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  mRNA Detection Kits

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Western Blot Signal Enhancer

  Western blot (immunoblotting) is widely used to identify specific proteins recognized by polyclonal or monoclonal antibodies. To detect low level expressed proteins successfully, it is crucial to increase the sensitivity of detection. Vicgene’s proprietary Western Blot Signal Enhancer reagent is designed to meet this requirement. Western Blot Signal Enhancer strongly promote antigen and antibody interactions and offers ultra high signal-to-noise ratios, resulting in outstanding sensitivity and low background.

4ug (Lane A) or 1ug (Lane B) of A431 whole cell lysis is immunoblotted with p53 antibodies (Pab1801 and DO-1) diluted with either Blotto (blotto panels) or Signal Enhancer (Signal Enhancer panels)

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siRNA Products

  Small interfering RNA (siRNA) is a short double strand RNA, which hybridizes to the target RNA and directs target degradation by enzymatic cleavage. Vicgene provides siRNA products targeting hundreds of different genes.  They are supplied in DNA forms with antibiotics selection marker to facilitate establishing stable transfected cell lines in very short time.  The constructs also contain special DNA sequences to enhance the integration of the entire constructs into host cell chromosomes in  high frequency.  To quantitatively assay gene silence efficiency, Vicgene also provides Gene Expression Quantitative Kits to the corresponding siRNA products

 

 

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                                siRNA Cloning Kit

  The siRNA Cloning Kit is designed for inducing small interference RNA(siRNA) in mammalian cells driven by a humanU6 RNA polymerase III promoter.  The PsiRNA vector in the kit is pre-linearized to facilitate directional cloning.  The advantages of the product are:

• Directional cloning synthesized oligonucleotides into the

  vector

• No restriction enzymes needed

• No PCR needed to avoid mutations

• Low non-recombinant background; >95% recombinants

• Simple protocol

• Transient expression of siRNA in mammalian cells for

  quick cell function assays or

• Very fast establish of stable siRNA expression cell lines 

   for long term cell  function studies

 

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Chemokine Products

  Vicgene is also devoted to provide the life science research community with the high quality chemokines.

 

 

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