siRNA Products

  Small interfering RNA (siRNA) is a short double strand RNA, which hybridizes to the target RNA and directs target degradation by enzymatic cleavage. Vicgene provides siRNA products targeting hundreds of different genes.  They are supplied in DNA forms with antibiotics selection marker to facilitate establishing stable transfected cell lines in very short time.  The constructs also contain special DNA sequences to enhance the integration of the entire constructs into host cell chromosomes in  high frequency.  To quantitatively assay gene silence efficiency, Vicgene also provides Gene Expression Quantitative Kits to the corresponding siRNA products

                                siRNA Cloning Kit

  The siRNA Cloning Kit is designed for inducing small interference RNA(siRNA) in mammalian cells driven by a humanU6 RNA polymerase III promoter.  The PsiRNA vector in the kit is pre-linearized to facilitate directional cloning.  The advantages of the product are:

• Directional cloning synthesized oligonucleotides into the

  vector

• No restriction enzymes needed

• No PCR needed to avoid mutations

• Low non-recombinant background; >95% recombinants

• Simple protocol

• Transient expression of siRNA in mammalian cells for

  quick cell function assays or

• Very fast establish of stable siRNA expression cell lines 

   for long term cell  function studies